Optics Controls allow you to manipulate the microscope's optics while manually inspecting a plate. The optics controls appear on the left-hand of the Live Image Sub-Tab of the Imager Tab, and vary based on the imaging methods you purchased.
There are six possible optics control panels, as shown below.
| Visible Light Optics Controls
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UV Optics Control
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| FRAP Optics Controls
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SONICC Optics Controls
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| SLP Visible Light Optics Controls
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SLP UV Optics Controls |
Use the table below to learn about the various controls and what effects they have on the resulting live image.
| Control | Imaging Methods | Description | Notes |
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| Zoom | - Visible - UV - FRAP - SONICC |
The Zoom field populates with the current zoom setting and is related to the FOV field. These fields allow you to zoom in on regions of interest within a drop or well. | Available zoom settings depend on the options you purchased. In most cases the zoom selection is a physical change of the objectives. In SONICC, changing the zoom value changes the laser beam scan angle. |
| Field of View (FOV) | - Visible - UV - FRAP - SONICC |
You can use the FOV fields to manually set the size, in µm, of the image you would like to view. As you change the FOV value using either the text entry field or the arrows, the zoom value automatically adjusts. | |
| Bright Field | - Visible - FRAP |
Bright field illumination passes light through the specimen under the microscope so that the specimen appears dark against a bright background. At 0%, the bright field light is turned off, and at 100% the light is at maximum brightness. | |
| Condenser | - Visible - FRAP |
The condenser collects light from the Köhler light source and concentrates it onto the well being examined. Values range from 0 – 100%. At 0%, the iris is fully open, and a cone of light is concentrated at a wide angle. At 100%, the iris is closed, and the light is concentrated in a column of light as opposed to a cone. Adjusting the condenser value can improve image contrast. Increasing the condenser angle increases the contrast. |
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| Polarizer | - Visible |
This field describes the angle at which the polarizer lens is projecting the light from 0 - 360°. Recommended settings are 0° or 90°. At 0°, you are not using cross polarized imaging. At 90°, you are using cross-polarized imaging. At 90°, if there is a crystal in the FOV, the crystal will light up due to the crystal's birefringence (if there is no crystal, the FOV will appear dark). | |
| Illumination | - Visible - UV - SLP UV |
On the visible imaging method, the illumination list provides you with various illumination patterns, including glancing, dark field, and bright field illumination. With the UV imaging method, the illumination button controls whether the LED UV light source is on or off. | |
| Laser | - FRAP | Controls whether the laser, which bleaches the sample, is on or off. | |
| Objective | - SONICC | The Objective control offers 10x, 20x or 40x magnification through the drop-down menu. Your selection affects Zoom and FOV options. | |
| Sensor | - SONICC | The Sensor selection determines whether you are using SHG or UV-TPEF. | The SHG imaging method images the sample with IR and detects the SHG (532 nm) in transmission. Signal is only generated from chiral crystals. The UV-TPEF imaging method images the sample with green light (532 nm) and detects the two photon fluorescence generated (350-400 nm). Signal is generated from UV-fluorescent molecules like tryptophan. |
| Fluoro Light | - FRAP | The fluoro light is the florescent light source. Changing this value affects the fluorescence signal intensity. 100% is the most intense. |
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| RIC-V216R216 |